This event is designed to help new faculty (those within their first five years of appointment) and postdocs network, learn, and find support. In the past, topics covered in this event included tools and techniques for managing budgets effectively, tips for negotiating and establishing relationships with vendors, and tips on being a supportive mentorAdvance registration is required.
Catharine Rankin, University of British Columbia, The success of the Canadian Rare Disease Models and Mechanisms program
Todd Lamitina: Lost in translation: new C. elegans models of repeat expansion disorders
Oliver Blacque: Interpreting ciliopathy patient mutations using C. elegans knock-in models
Hiba Daghar, U. of Montreal, Developing C. elegans models for SRD5A3-CDG and Cori rare congenital diseases
Peter Kropp, NIDDK/NIH, Parsing allele-specific effects of a mitochondrial disease gene
COPAS VISION is a flow cytometer that can analyze and sort all stages of C.elegans and collect brightfield images of those worms. This lets the researcher screen through populations for rare variants, selecting differences in fluorescence levels, and dispensing worms to wells for various assays. All this and worm snapshots!
Also be sure and visit us at the Poster and Exhibits sessions on Monday, Tuesday and Wednesday.
This workshop is dedicated to technological advances that allow precise control of gene expression and protein abundance. Ground breaking work by Andrew Fire and Craig C. Mello on RNAi as a potent tool to silence gene expression has had a tremendous impact on the C. elegans field and beyond. Nevertheless, additional layers of manipulation are important to obtain experimental alternatives that often provide faster, more precise and/or reversible regulation of gene activity. Leading researchers involved in the development of tools for drug inducible gene expression, genome recombination and targeted protein degradation and localization will share their recent advances and experience with the audience through open discussion.
11:30 a.m. Introduction by Jordan D Ward, University of California-Santa Cruz
11:34 a.m. Mike Nonet, Washington University School of Medicine, RMCE and RMHE integration approaches and bipartite expression systems
11:41 a.m. Mohammed Al Johani, King Abdullah University of Science and Technology, Efficient germline expression of transgenes
11:48 a.m. Justin Shaffer, Columbia University, FLExon: a FLoxed Exon approach to conditional gene expression
11:55 a.m. Lloyd Davis, University of Edinburgh, Controlling Gene Expression with Light
12:02 p.m. Peter Askjaer, Andalusian Centre for Developmental Biology, Expanding the FLP/Frt Toolkit
12:09 p.m. Theresa Gibney, University of Virginia, Genome engineering methods to visualize and manipulate endogenous proteins with cell-type specificity
12:16 p.m. Maria Sallee, Stanford University, Tissue-specific degradation of endogenous proteins using the ZIF-1/ZF system
12:23 p.m. Kelly Hills-Muckey, Cold Spring Harbor Laboratory, Auxin-TIR1 pair mutation improves efficacy and specificity of the Auxin Induced Degron (AID) system
12:30 p.m. Open discussion
11:45 a.m. Chris Grove, California Institute of Technology, Researching transcriptional regulation using WormBase transcription factors, TF binding sites and the modENCODE data
12:00 p.m. Ranjana Kishore, California Institute of Technology, Comparative genomics and disease research using Alliance of Genome Resources
12:15 p.m. Daniela Raciti, California Institute of Technology, How can you contribute? Community curation and tools, and the author-first-pass (AFP) pipeline
12:30 p.m. Chris Grove, California Institute of Technology, Open Discussion / Q & A
This great new era of C. elegans natural biology has unearthed a new field in the community dedicated to understanding the role that microbes have played in sculpting the physiology of our beloved model system. In the wild, microbes not only act as potential food or pathogen, but can also colonize the intestines of C. elegans in simple communities (‘microbiomes’). Interest in this field has exploded since the first descriptions of these communities in wild C. elegans and introduction of the characteristic core microbiome in the first workshop, yet there is still great opportunity ahead. The aim of this third workshop is to provide an overview of this emerging field and the evolving directions, to facilitate cross-fertilization between the different approaches, and to introduce members of the C. elegans community to useful research pipelines and available resources.
C. elegans researchers share the work they’re doing to address the lack of diversity within our field at various scientific stages. This session aims to include talks from individuals working at increasing diversity at the undergraduate research level through the professoriate. Our intention is that this session will generate communication within the community, spur individual ideas and actions, and express our plans to continue facilitating these conversations at future Worm meetings. We hope that highlighting these topics communicates that building a diverse, equitable, and inclusive scientific enterprise should be a priority for all scientists, and we want to give our community concrete ideas to take back to the classroom and the lab.
Are you interested in learning to teach more effectively? Would you like to make your classroom more inclusive and engaging? Could you use guidance on how to implement active learning in your classes? Learn about the PALM Network (Promoting Active Learning and Mentoring), funded by the NSF and sponsored by the GSA and ASCB. This workshop is led by a current PALM Mentor and a former PALM Fellow. We will examine the benefits of active learning strategies, highlight advantages of belonging the PALM Network, describe examples of PALM projects, and discuss how to craft a successful application.
NemaLife, Inc invites you to experience how our hardware and software solutions can help improve the experimental throughput of your lab. We will demonstrate how our microfluidic platforms reduce the need for intensive manual assays. We will also highlight how our new software tools can speed up data analyses. Retire your worm picks with us! Stop by and visit us during the Poster and Exhibit sessions on Monday, Tuesday and Wednesday!
11.30 a.m, Scott W. Emmons, Albert Einstein College of Medicine, Studies on the C. elegans male, how we got to where we are today
11.45 a.m. Arantza Barrios, University College London, Switching odour preferences through neuromodulation
12.00 p.m. Vladislav Susoy, Harvard University, Brain-wide functional analysis of mating behavior
12.15 p.m. Tessa Marie Tekieli, Columbia University, Visualizing the organization of the male-specific nervous system of C. elegans
12.30 p.m. Chen Wang, Columbia University, Mutant analysis of the DM-domain transcription factors using C. elegans male gene expression atlases
12.45 p.m. Questions from the audience
Imaging single molecules in intact cells has the potential to reveal features of gene expression that are not possible to measure using standard, ensemble-based strategies. While a number of model organisms have successfully employed aptamer-based transcript imaging systems (MS2, PP7, etc.) to track individual RNAs in real time, these approaches have had only limited success in C. elegans. This workshop intends to build momentum toward establishing these systems throughout C. elegans research community which will complement this powerful genetic model and enable aspects of RNA transcription, export, localization, translation, and turnover to be studied in detail.
11:30am Introduction: C.M. Hammell (CSHL), Sevinc Ercan (NYU, and Erin Osborne Nishimura (CSU).
11:35am ChangHwan Lee (SUNY Albany), “Capturing dynamics of transcriptional bursting in vivo using the MS2 system.”
11:55am Hongjie Zhang, Universidade de Macau, “PP7/PCP-based visualization of membrane-associated transcripts in epithelia.”
12:15pm Wolfgang Keil, Curie Institute, “Monitoring spatiotemporal patterns of post-embryonic miRNA transcription using the MS2 system.”
12:35pm Erin Osborne Nishimura, Colorado State University, “Best practices in mRNA live imaging.”
12:45pm General Discussion and Panel Questions.
Ellen Gregory from UC Davis and Dr. Ken Dawson-Scully from Florida Atlantic University will discuss how humanized C. elegans models and novel assays are used for disease modeling with the goal of assaying the clinical significance of predicted disease-causing variants and for uncovering neurotoxins and biowarfare antidotes.
Each year infections of animals and plants by parasitic nematodes cause many billions of dollars of agricultural damage. Over 1.5 billion people worldwide, particularly in developing nations, are infected by nematodes and suffer from the resulting debilitating diseases. Currently, only a few investigators address problems of parasitic nematodes using C. elegans. To encourage and facilitate more interactions between the C. elegans and parasitic nematode communities, workshops have been held for experts in plant, animal and human parasitic nematodes to speak on the life history and unique biology of these parasitic species and on outstanding issues in their field. A key goal of this workshop is to make C. elegans scientists aware of the issues and problems that parasitic nematode researchers face and pave the way for applying the powerful approaches and technologies that have advanced C. elegans research to parasitic nematodes.
3:30 p.m. - 4:00 p.m. Vicky Hunt, piRNA-like small RNAs target transposable elements in the clade IV parasitic nematode Strongyloides ratti
4:00 p.m. - 4:10 p.m. Kyriaki Neophytou, Elucidating the interaction partners of an extracellular Argonaute protein
4:10 p.m. - 1:20 p.m. Astra Bryant, Parasite-specific encoding of thermosensory signals by the human threadworm S. stercoralis
4:20 p.m. - 4:30 p.m. Sophia Parks (Dillman lab), Parasitic nematode fatty acid- and retinol-binding proteins compromise host immunity by interfering with host lipid signaling pathways
4:30 p.m. - 4:40 p.m. Break, Q&A.
4:40 p.m. - 5:10 p.m.. Louise Atkinson, Advances in Nematode Parasite Omics Seeding Drug Discovery Pipelines
5:10 p.m. - 5:20 p.m. Stephen Doyle, Improving parasite genomes in the post-genome era
5:20 p.m. - 5:30 p.m. Jonathan Stoltzfus, Utilizing transcriptomics to examine dauer and sex determination pathways in the human parasitic nematode Strongyloides stercoralis
5:30 p.m. - 5:40 p,m.. Break, Q&A.
5:40 p.m. - 6:10 p.m. Erik Anderson, The genetics of resistance in free-living and parasitic nematodes
6:10 p.m. - 6:20 p.m. Jessica Knox, Exploiting C. elegans and Tractable Parasitic Nematodes for the Discovery and Characterization of Anthelmintics and Nematicides
6:20 p.m. -6:30 p.m. Nate Schroeder/David Hall, Developing WormAtlas beyond C. elegans
6:30 p.m. - 6:45 p.m. Closing remarks