Heavy metals are naturally present on earth; nevertheless, their bioaccumulation in the environment triggers severe ecological issues, jeopardizing the ecological balance and ultimately producing detrimental effects on human beings and different living systems. To assess the genotoxic effect of various heavy metals, we used Drosophila third instar larvae polytene chromosomes as a model. Many chromosomal aberrations (breaks, semibreaks, constrictions and asynapsis) were observed. The frequency of chromosomal aberrations was significantly increased (P-value= 0.001) in groups treated with heavy metals at a concentration of 1000ppm compared to the control group. Understanding the genotoxicity of heavy metals is of great importance. Lack of such information could lead to difficulty in evaluating the danger of such environmental pollutants. For this reason, studying the various effects of these environmental contaminants on Drosophila melanogaster polytene chromosomes could aid in measuring their impact at the chromosomal level rather than going to the chemical analysis.