Argonaute family proteins are not only conserved in higher organisms but also found in archaea and bacteria (Aquifex aeolicus). The name Argonaute (Ago) came from the phenotypic resemblance of Arabidopsis thaliana mutants with mollusk ‘Argonaut’. PAZ and PIWI domain containing argonaute family proteins are well known for their role in RISC complex of RNAi pathway. Role of Ago-1 in micro RNA biogenesis has been thoroughly studied, but its role in mitotic cell cycle progression is not studied much. In our earlier observation we have established the role of Ago-1 in cell cycle control in association with G2/M cyclin. We have further extended our study in understanding the relationship of Ago-1 in the regulation of apoptosis during Drosophila development. Apoptotic cell death plays a very critical role during development of multi cellular organism. Apoptotic process is controlled by multiple regulatory pathways. In this study we have used Drosophila eye as the phenotypic marker in producing overexpression of Ago-1 by employing the unique UAS-GAL4 system under the expression of eye specific promoter. Overexpression of Ago-1 in the eye resulted in smooth-eye phenotype with reduced number of ommatidia along with fused ones and loss of bristles in eye area. By combining a single copy of the dominant negative basket (Drosophila homolog of JNK) gene mutation, we found a drastic reversal to the normal phenotype indicating an active involvement of the basket with Ago-1 induced developmental apoptotic process. Pro-apoptotic genes hid, grim & rpr and Drosophila caspase genes, dronc, ice & Dcp-1 are also involved in this machinery. Thus Ago-1 regulated developmental apoptotic process in Drosophila development is JNK dependent.