Chemical synapses are specialized structures to mediate communication between neurons and their target cells through neurotransmitter release mechanism. The transport, docking and release of neurotransmitters heavily rely on synaptic vesicles. Therefore the control of synaptic vesicle transport and localization is of great importance in the function, development and maintenance of nervous system. Here, we report that the C. elegans liprin protein SYD-2 is required for synaptic vesicle transport and localization. Mutations on syd-2 lead to ectopic synaptic vesicle distribution, especially to the distal axon. syd-2 regulated synaptic vesicle localization is dependent on kinesin/UNC-104. Previous study showed SYD-2 can physically interact with UNC-104, and UNC-104::GFP retrograde transport is increased in syd-2 mutants. However, systematic genetic analysis implies that SYD-2 may recruit synaptic vesicles,which is independent from UNC-104-interaction. Overall, our study reveals an important role of active zone protein SYD-2 in synaptic vesicle localization.