The feijoa fruit (Feijoa sellowiana) is a rich source of bioactive compounds such as tannins, terpenoids, alkaloids, and flavonoids. Of the 95 compounds reported thus far, these compounds exhibit a diverse suite of bioactivity including antimicrobial, antifungal, anticancer, and antiviral activity. To characterize the genetic and cellular mechanisms of action of two potentially novel compounds derived from feijoa fruit, we used Bar-seq analyses to identify the genes and pathways mediating the bioactivity of these compounds. Next-generation sequencing was used to sequence the barcodes in the heterozygous and homozygous diploid libraries. The barcode sequences were mapped to the reference barcodes and differential abundance analyses were used to compare barcode abundance in treated and untreated genomic libraries. The least represented genes in the heterozygous diploid library will be identified as candidate primary targets of the compounds, while the least represented genes in the homozygous diploid library will be identified as candidate targets downstream of the primary target. The results of our Bar-seq analyses will be presented at the meeting via experiments in yeast and mammalian cells. .