Similar to human, the intestine of C. elegans contain and process constantly E.coli bacteria since it fed on bacterial lawn. This observation led us to consider the bacterial lawn of C. elegans not only as a food source but as a microbiota environment. Subsequently, we can modify by genetic engineering this environment to study host-pathogen interaction in the gut of the nematode. Since a nodavirus in C. elegans called orsay was recently discovered we validated this method using this new viral system. In this study, we developed a novel method called PROtein FEeding in C. Elegans (PROFECE) that fed the worm with protein expressed in the E. coli bacterial lawn. We further investigated the effect of the viral proteins alpha and delta during development using survival assay, fluorescence microsocopy and behavioral assay with a multi-wormtracker. Survival assays shows that the viral proteins affect lifespan and provoke intestinal malfunctions and abnormalities. Finally, we observed using wormtrackers that worm fed with E. coli lawn expressing different viral proteins have behavior that is similar but different from the two well described mode of locomotion defined as roaming and dwelling in response to different type of food likelihood. Our results suggest that nematode growing in the presence of the orsay viral proteins have an altered muscular and/or neuronal function.