After a meal, digestion occurs and nutrients are absorbed through the walls of the intestine and transported throughout the body to provide energy for cellular functions. Excess energy is stored as triglycerides and glycogen. Several enzymes act to break down complex macromolecules so they can be absorbed through the intestinal epithelia. However, the full complement of genes that function in the intestine to regulate nutrient absorption and storage are not fully understood. Carbohydrate response element binding protein (ChREBP) is a transcription factor known to function in the liver as a nutrient sensor that stimulates triglyceride storage in response to elevated nutrient levels. In addition to being expressed in the liver, ChREBP expression is enriched throughout the small intestine; however, whether ChREBP functions in the intestine to regulate nutrient absorption or storage is unknown. The Drosophila genome contains a single ChREBP homolog known as Mondo, and our lab has shown that this gene functions in the fly brain, muscle, and fat body to regulate feeding, nutrient storage, and muscle function. To test whether Mondo functions in the intestine, we measured levels of triglycerides and glycogen in flies where Mondo has been decreased specifically in the intestine using RNAi. We have found that lowering Mondo levels in the intestine results in a decrease in triglyceride and glycogen levels. This altered nutrient storage phenotype does not seem to be due to less nutrient intake as food consumption is not altered in Mondo-knockdown flies. Together, these data suggest that Mondo functions in the fly intestine to regulate nutrient absorption or transport in response to nutrient availability. .