Many industrial chemicals, such as pesticides, are released into the environment without a full assessment of their adverse effects on human life and the ecosystem. We aim to understand the developmental toxicity effects of environmental pollutants by using the vertebrate zebrafish (Danio rerio) as a model to identify neuromast disruptors. The zebrafish lateral line responds to the flow of water via receptors called neuromasts, which consist of a cluster of hair cell surrounded by a ring of supporting cells and innervated by sensory neurons. Neuromasts are positioned along the zebrafish body through migration of the neuromast primordium formed in the head region. The same family of chemokine receptors that are responsible for neuromast migration also guide metastasis of certain cancer cells (Galardo et al., Disease Models & Mechanisms (2015) 8, 565-576). Transgenic zebrafish embryos expressing fluorescent markers in neuromasts were used to visually monitor neuromast development under normal and perturbed conditions using the control compounds AG1478 and VPA. Next, these embryos were used in a primary high throughput screen (HTS) of 309 (294 unique) compounds of the ToxCast phase I chemical inventory, primarily consisting of pesticides and antimicrobials. This generated a list of 48 hits that altered neuromast development and/or migration. These hits were re-screened with a higher number of replicates to characterize effects and to determine the lowest effect levels (LELs). A univariate analysis was performed to identify ToxCast in vitro assays that significantly correlated with the identified in vivo neuromast disruptors. In addition, the effect of IT1t, which has been shown to inhibit the metastasis of MDA-MB-231 cells in vivo (Tulotta, C. et al. Disease models & mechanisms (2016) 9, 141-153), was tested on neuromast migration. To further investigate the connection between cancer metastasis and neuromast migration, we investigated the effects of neuromast disruptors on the migration of MDA-MB-231 cells. In conclusion, our results identified several environmental pollutants with neuromast disrupting capacity, suggesting that the developing zebrafish embryo is an efficient in vivo model that can be used for identification of compounds interfering with cell migration.