PgmNr D1054: Transcriptome analysis to identify genes responding to mechanical force in developing Drosophila embryos.

Authors:
T. Ishibashi 1 ; K. Yamaguchi 2 ; S. Shigenobu 2 ; K. Matsuno 1


Institutes
1) Osaka University, Toyonaka, Osaka, JP; 2) National Institute for Basic Biology, Okazaki, Aichi, JP.


Keyword: morphogens

Abstract:

Various types of mechanical force are involved in biological processes. Especially during development, cells are subjected to dynamic mechanical force. Cells growing in limited area push and pull their neighboring cells, which may change the shape of the tissues encompassing or surrounding these cells. Recently, it has been reported that mechanical force also modulates cell behaviors, such as polarization, cell migration, cell growth, and differentiation. However, most of these studies rely on cultured cells. Therefore, it is largely unknown what molecules are involved in generation and reception of mechanical force, and how they work in developmental events.
  In order to reveal these issues, we planed to perform RNA-seq transcriptome analysis to identify genes induced by mechanical force in D. melanogaster embryos. First, we constructed the mechanical force application system. We confirmed that the compression during the whole embryogenesis perturbed development, however the compression for 30 minutes did not affect the hatching rate. Next, we checked the global stress response to the compression. We confirmed that ER-stress response was not affected by the compression for 30 minutes.
  Here, we will describe our experimental system and discuss our future directions.