Zebrafish are an important model for investigating vertebrate circadian clocks. However, scarce of zebrafish genetic circadian mutants has hindered further advancement of the zebrafish circadian field. We set out to dissect molecular genetic mechanisms underlying zebrafish circadian clocks. Using retroviral insertional mutagenesis, TALEN or CRISPR-Cas9, we have generated a library of zebrafish circadian mutants. Here we report characterization of zebrafish per1b, per2 and rev-erbα mutants. Behavioral assays showed that under light/dark (LD) condition, per1b mutant larvae are approximately three times more active than their wild-type counterparts and the amplitude of rev-erbα mutants increases more than 40% during the whole duration of a 24-hour day and night, while per2mutant larvae have approximately 30% reduced activity amplitude in comparison with wild types; under constant darkness (DD) condition, locomotor activity of per1b mutant larvae is phase advanced by two hours, while locomotor activity of per2 mutant larvae is phase delayed by two hours, and rev-erbα mutants display 0.5-hour phase delay and 0.25-hour shortened period. Under DD condition, per1a, per2 and per3 were all significantly up-regulated in per1b mutants, indicating that Per1b serves as a negative regulator in the zebrafish circadian system. Under LD condition, bmal1a and annat2 were significantly up-regulated but per1a, per1b and bmal1b were significantly down-regulated in the per2 mutant fish. In Vitro cell transfection assays showed that Per2 not only acts through E-boxes to repress E-Box-containing genes but also acts through RRE-boxes to activate RRE-box-containing genes. Under DD condition, both bmal1b and bmal2 are significantly up-regulated in rev-erbα mutant larvae. We also found that zebrafish per1b mutants display hyperactive-, impulsive-, and attention deficit-like behaviors and low levels of dopamine, reminiscent of human Attention Deficit Hyperactivity Disorder (ADHD) patients. Rev-erbα modulates autophagy rhythms directly via controlling expression of autophagy and autophagy-related genes, and also plays regulatory roles in stress response, metabolism, signal transduction and posttranslational modification. Taken together, our findings demonstrate that Per1b, Per2 and Rev-erbα are essential for the zebrafish circadian clock and also play regulatory roles in numerous fundamental life processes, and highlight zebrafish as an attractive model for investigating vertebrate circadian rhythmicity.