PgmNr D133: E-Cadherin decrease transmits proliferative-dependent forces via actin-myosin flows.

Authors:
D. Pinheiro 1,2 ; E. Hannezo 3 ; S. Herstzerg 1,2,4 ; I. Gaugue 1,2 ; M. Balakeriva 1,2 ; Z. Wang 1,2 ; S. Rigaud 1,2 ; O. Markova 1,2 ; Y. Bellaiche 1,2


Institutes
1) Institut Curie, Paris, FR; 2) Sorbonne Universités, UPMC, France, Fr; 3) Cavendish Laboratory, Department of Physics, Cambridge, UK; 4) The Francis Crick Institute, Mill Hill Laboratory, London, UK.


Keyword: cell junctions and adhesion

Abstract:

During epithelial cytokinesis, the remodelling of adhesive cell-cell contacts between the dividing cell and its neighbours has profound roles in the integrity, arrangement and morphogenesis of proliferative tissues. This remodelling is powered by an interplay between contractile ring cosntriction in the dividing cell and the dynamics of non-muscle Myosin II (MyoII) in the interphasic neighbouring cells. Here we explore how the dividing cell regulates MyoII dynamics in its neighbours. We found that pulling forces, resulting from contractile ring constriction, lead to MyoII accumulation in the neighbouring cells independently of the classical Vinculin-mediated mechano-transduction pathway. We established that the contractile ring pulling forces promote local junction elongation, resulting in a decrease of E-Cadherin concentration at the ingressing adherens junction (AJ). In turn, the local reduction of E-Cadherin concentration and the contractility of the neighbouring cells promote a self-organized outward flow of F-Actin and MyoII, ultimately leading to their accumulation at the base of the ingressing AJ. Mechano-sensing has been extensively studied in the context of AJ strengthening to stabilize adhesive cell-cell contacts, we propose an additional mechano-sensing mechanism able to coordinate actin-myosin dynamics between epithelial cells and to sustain AJ remodelling in response to mechanical force..



Flybase Genetic Index:
1. FlyBase gene symbol: shg
2. FlyBase gene symbol: sqh
3. FlyBase gene symbol: Vinc