PgmNr W4117: Genome engineering with the CRISPR/Cas9 system in C. elegans.

Authors:
Daniel J. Dickinson; Bob Goldstein


Institutes
Department of Biology and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC.


Keyword: Genome editing

Abstract:

The CRISPR/Cas9 system is fast becoming an indispensible tool for research in model organisms, including the nematode C. elegans.  In just the past few years, a large number of different strategies for CRISPR in C. elegans have been described.  The purpose of this presentation is to aid users in choosing the most effective strategies for their applications.  We will summarize our own contributions to genome editing techniques for the worm, along with the work of others, and provide recommendations for which approaches to try first for different types of genome modifications. In addition, we’ll present some novel applications and improvements to our selection-based protocol (Dickinson et al. 2015), including improved cloning methods, a streamlined approach for structure-function experiments, and a conditional balancer strategy that facilitates functional studies of essential genes.