Ingestive behavior is composed of parameters such as meal frequency and meal size. Previous studies suggest that increased meal size plays a large role in the propensity for obesity. Thus, understanding how meal size is regulated could lead to interventions to treat obesity, diabetes, and general eating disorders. The advent of novel methods for measuring Drosophila feeding behavior has increased its use as a model for understanding the mechanisms underlying the regulation of hunger and satiety. Meal size is thought to be dictated by oral and post-ingestive signaling; however, the interaction between the two remains unclear. We found that feeding flies increasing sucrose concentrations resulted in decreased average meal size. In contrast, increasing concentrations of sucralose, a non-nutritive but sweet tasting substance, increased meal size. Interestingly, following starvation, meal size had a non-monotonic relationship with sucrose concentration, revealing state-dependent positive regulation by sucrose. These data suggest that accumulating hunger may unequally modulate taste and post-ingestive signaling to determine meal size. Furthermore, we have identified a subset of neurons that are necessary for the regulation of meal size; when these neurons are silenced, animals are unable to regulate feeding and meal size is increased. Future studies will determine whether these neurons regulate oral or post-ingestive signaling and will further elucidate the mechanisms underlying meal size regulation.