Alcohol use disorder (AUD) imposes adaptations that perturb psychological and physiological characteristics of an individual that can contribute to the addicted state. Drosophila are favorably used to study alcohol-related behaviors due to their well-developed genetic toolkit, and shorter lifespans. However, previous researchers have struggled to quantify alcohol consumption in Drosophila due to their small size. In addition, the assays available currently cannot measure consumption in a temporally robust way, and rely on starvation to motivate the animals. We have developed a novel assay that measures the amount of consumed ethanol and can accurately track alcohol preference in a starvation independent paradigm. This new assay is temporally precise and better emulates natural feeding behaviors. Using this assay we have found male Canton S Drosophila naïve to ethanol are innately aversive to it as a food source (>5% ABV), however this aversion disappears when the flies are pretreated with ethanol. In addition, female Drosophila have innate attraction to ethanol (<15% ABV). Now, we are measuring ethanol preference in mutants that were selected through identifying genes that were epigenetically regulated during tolerance acquisition. These mutants are not able to acquire functional tolerance to alcohol and testing their ethanol preference will determine if tolerance to alcohol exacerbates preference for drinking.