Li-Ping Shu * Feng Liu * Hui-Min Huang, Heng-Lu Zhang, Xi-Jun Wu, Jun Wang, Zhi-Xu He #( * co-author # correspongding author(hzx@gmc.edu.cn))
Abstract:objective Lamins are the major components of nuclear lamina underneath the inner nuclear membrane (INM). Lamins express in most cell and involve in the whole process of growth, which play a major role in cell stability and embryo development. While the effect on lymphoid transcriptional regulation factors correlate with the downregulation of lmna during hemotopoiesis remains unknown. method MO(Morpholino Oligomer) tagged to zebrafish lmna gene was microinjected into zebrafish embryos of different phases to downregulate the expression of lmna. The expression level of different transcriptional regulation factors such as lmo2, scl,c-myb and runx1 were detected by Q-PCR. result The Q-PCR results show that the expression of transcriptional regulation factors lmo2 and scl, which associated with primitive hemotopoiesis were downregulated in 18h, 24h, 30h and 36h groups compared with control group. c-myb and runx1 were also downregulated. conclusion These results revealed that lmna may have a relationship with hemotopoietic transcriptional regulation factors and have a negative effect on embryo development.
Acknowledgments
This work was supported by the National Natural Science Foundation of China (NO. 31260284). We thank all members of the Department of Immunology and the Center of Stem Cell and Tissue Engineering Research for discussions and valuable comments.