PgmNr D242: Niche Appropriation by Drosophila Intestinal Stem Cell Tumors.

Authors:
P. H. Patel 1,2 ; D. Dutta 1,2 ; M. Roca 1,2 ; B. A. Edgar 1,2


Institutes
1) German Cancer Research Center (DKFZ), Heidelberg, Germany; 2) Center for Molecular Biology, University of Heidelberg (ZMBH), Heidelberg, Germany.


Keyword: niche and other local signaling

Abstract:

Mutations that inhibit differentiation in stem cell lineages are a common early step in cancer development, but precisely how a loss of differentiation initiates tumorigenesis is unclear. We investigated Drosophila intestinal stem cell (ISC) tumors generated by suppressing Notch (N) signaling, which blocks differentiation. Notch-defective ISCs require stress-induced divisions for tumor initiation and an autocrine EGFR ligand, Spitz, during early tumor growth. Upon achieving a critical mass these tumors displace surrounding enterocytes, competing with them for basement membrane space and causing their detachment, extrusion and apoptosis. This loss of epithelial integrity induces JNK and Yki/YAP activity in enterocytes and, consequently, their expression of stress-dependent cytokines (Upd2, Upd3). These paracrine signals, normally used within the stem cell niche to trigger regeneration, propel tumor growth without the need for secondary mutations in growth signaling pathways. Interestingly, we have recently found that tumor growth additionally induces p38 signaling in surrounding epithelial cells. The appropriation of niche signaling by differentiation-defective stem cells may be a common mechanism of early tumorigenesis.