PgmNr P385: How much do chromosomal inversions prevent gene conversion and interspecies gene flow?

Authors:
Katharine Korunes


Institutes
Duke University, Durham, NC.


Abstract:

Many distinct species continually hybridize, and the persistence of these species is attributed in part to recombination barriers such as chromosomal inversions. Chromosomal inversions are proposed to act as barriers to recombination in inversion heterozygotes, but the strength of these barriers remains poorly understood. While single crossover products are prevented within inversions, non-crossover gene conversion can occur in and around chromosomal inversions, potentially even at high rates, rendering inversions less effective at preventing gene flow. There are extremely limited empirical estimates of non-crossover gene conversion rates with respect to chromosomal inversions, so despite the prevailing thinking, inversions may be quite ineffective at keeping hybridizing species distinct. To test the efficacy of chromosomal inversions at maintaining LD in hybrids, I am utilizing the naturally-hybridizing species pair Drosophila pseudoobscura and D. persimilis. Using whole-genome sequence data from experimental crosses, I detect gene conversion rates within inverted regions of species hybrids that are at least as high as rates based on within-species LD data. With these data, we can assess rates of gene flux and better understand the conditions under which chromosomal inversions reduce interspecies gene flow. Given such high rates of gene conversion in hybrids but high sequence differentiation between species, other features of this system must be maintaining the distinction of these species besides the recombination barrier provided by chromosomal inversions.