Nutritional stress is known to block stem cell multiplication and cause delay germ cell divisions. In the present study nutritional regulation of oogonial proliferation and differentiation into primary oocytes was studied in zebrafish (Danio rerio) by subjecting adult fish to different feeding regimes. Adult female zebrafish (wild) after acclimatization to the laboratory (temperature: 26 ± 1°C and photoperiod: 11.30L: 12.30D) for one week, were divided into five groups and maintained under different feeding regimes. Group-I daily four times fed; Group-II daily twice fed; Group-III daily once fed; Group-IV every alternate day fed and Group-V every fourth day fed. The experiment lasted for two months; all experimental fish were fed on commercial pellets and Artemia nauplii ad libitum. At the end of experiment, body mass and body size of each fish were recorded, ovaries were excised, weighed and processed for histology. Serial sections (3µm thick) were cut and stained with hematoxylin and eosin. Oogonia, primary oocytes, vitellogenic oocytes and atretic follicles were quantified from serial histological sections. Our observations reveal that there was an increase in body mass, ovary mass, number of oogonia and primary oocytes in daily four times, daily twice and daily once fed fish over initial controls. While they remained comparable to initial controls in alternate day fed fish, and were significantly lower in every fourth day fed fish. These results indirectly suggest that overfeeding increased body mass of fish without causing a corresponding increase in body size, oogonial population and their rate of proliferation while, underfeeding depleted oogonial number. It is concluded that nutrition regulates germ cell mitosis optimally in zebrafish ovary, overfeeding may not increase rate of proliferation linear but under feeding curtails it.