Visual impairments affect 285 million people worldwide: 39 million people are blind and 246 million people have low vision. Several lines of evidence suggest that it may be possible to restore vision by regenerating photoreceptors and neural connections. However, as novel methodologies are developed, a critical question will need to be addressed: how do we monitor in vivo for functional success? One approach to monitor for functional success in animal model systems is the analysis of behavior, since behavioral analyses can reveal subtle functional defects, even if the visual system appears normal by morphological criteria. The current study is focused on the automated analysis of behavior in response to visual stimuli, using zebrafish larvae as a model system. Zebrafish larvae are ideally suited for such studies, since high-throughput analyses of behavior can be combined with genetics, high-resolution imaging and experimental manipulations. We developed a novel behavioral assay using colored visual stimuli and found that 5-7 day-old zebrafish larvae display distinct responses to the visual stimuli. These behavioral responses were inhibited by photoablation of photoreceptors, but recover over time as the eye regenerates. The developed tools may be used in future research to screen small molecule libraries for novel treatments of blindness and low vision.