In China, zebrafish was adopted in lab research in early 1990s, and the zebrafish research community has been rapidly growing since 2000. To meet the growing needs of zebrafish strains and technical support, in October 2012, the China Zebrafish Resource Center (CZRC, website: http://zfish.cn) was established at the Institute of Hydrobiology (IHB), Chinese Academy of Sciences (CAS) in Wuhan. After three years’ development, CZRC has maintained more than 1000 mutant and transgenic zebrafish lines, and a number of DNA plasmids and cell lines tillnow. All the fish lines provided by CZRC are tightly linked to the web information of Zebrafish Information Network (ZFIN). In addition to providing the regular fish resources, CZRC also provides transgenic service, knock-out service and technical training. In 2013, 40 labs in China started the Zebrafish All Gene Knock-out Project (ZKO Project) for Chromosome 1. The final goal of ZKO project is to knock-out all the 1333 genes on zebrafish chromosome 1 using CRISPR/Cas9 technology. Up to now, more than 900 genes have been successfully knocked out. CZRC is the project coordinator, and all mutant lines built in the project will be verified and preserved by CZRC and published as open resources to the whole research field. With the progress of ZKO project, CZRC will expand its fish stocks to 2000 lines by the end of 2016, and it is becoming one of the major resource centers in the global zebrafish community.