PgmNr D1193: Identification and Characterization of Novel Genes in the Drosophila Heart.

Authors:
TyAnna Lovato; Dylan Brown; Devon Lagueux; Richard Cripps


Institutes
Univ New Mexico, Albuquerque, NM.


Keyword: cell differentiation

Abstract:

Drosophila is an effective model for understanding the genetic factors and pathways that direct the formation of the heart. Using fluorescent-activated cell sorting (FACS) of embryonic heart cells followed by RNA sequencing, we were able to identify novel genes expressed during embryonic heart development. In this project, we have chosen to characterize CG8147, CG9336, and CG7033. CG8147 has predicted alkaline phosphatase activity while CG7033 is a predicted chaperone protein with ATP binding capability. CG9336 currently has no known conserved functional domains. To understand the how these genes might affect heart development, we are utilizing CRISPR/Cas9 technology to generate mutant fly stocks in order to further investigate their role in development.  .



Flybase Genetic Index:
1. FlyBase gene symbol: CG9336; FBgn: FBgn0032897
2. FlyBase gene symbol: CG7033; FBgn: FBgn0030086
3. FlyBase gene symbol: CG8147; FBgn: FBgn0043791