Phenotypic plasticity allows individuals to, for example, adapt their physiological responses to their environment, as well as to the stage of their life cycle. Within a honeybee colony, worker bees start their adult life as nurses involved with building the honeycombs, feeding the larvae, and cleaning the hive. As such, nurse bees only need to fly limited distances and for short amounts of time. Some of the nurse bees transition later in life (around 21 days) to foragers whose job is to collect water and nectar, and as such foragers need to be excellent flyers. The nurse-forager transition event is a model system for understanding the molecular implication of phenotypic plasticity. This event necessitates major changes in the bee‘s behavior, but also in the efficiency and power generated by the flight muscle system. RNA sequencing analysis of flight muscle tissues isolated from nurse and foragers at different ages reveal differential gene expression of genes involved in several biological processes, including stress response, immunity, and protein synthesis. Proteins involved in muscle sarcomere structure, in particular proteins of the myofilaments undergo shift between isoforms during the transition. Quantitative RT-PCR analysis for proteins of the troponin complex, as well as the elastic C-filament will be presented. The generation of these alternative splice variants depends on the activity of regulatory splicing factors, such as muscleblind, lark, and others. The initial analysis of Apis mellifera splicing machinery and in particular regulatory splicing factors will be discussed in the context of the nurse-forager transition.