In order to decipher the contribution of glial cells in motor behavior and sleep regulation and to gain insight into glia-neural interaction, we used a “cell-centric” forward genetic screen approach to identify glia subset involved in these mechanisms. We hypothesize that specific glial cells are crucial for various key endpoints related to locomotor activity, arousal and sleep by modulating the functionality of specific neuronal subsections. We conducted an unbiased genetic screen by genetically manipulating subset of glia cells within a broad glial-specific repo-Gal4 expression pattern using the FINGR (Flippase-induced Intersectional Gal80/Gal4 Repression) method. We perturbed the function of specific glia subsets by expressing a UAS-effector (polyglutamine, polyQ) to determine its effect on behavior, and mapped glial morphology and location by labeling the relevant subset with GFP. We assayed locomotion, arousal and sleep in flies by using a video-based platform, and the DART (Drosophila Arousal Tracking) system in a 12:12 L:D cycle. An ongoing study has screened 122 lines (male and female) and identified 27 lines with a defect in sleep impacting daytime or nighttime sleep or both. We believe that the characterization of these sleep phenotypes should help identify specific and novel roles of subset glia modulating neurons in the regulatory mechanisms of sleep and its functional consequences.