Insulin signaling is generally involved in nutrient sensing and growth and the pathway, overall, is highly conserved from fruit flies to man. In many organisms insulin signaling frequently plays a role in control and development of sexually dimorphic traits, including both differences in growth and in behavior. In Drosophila, this pathway plays a direct role in regulating body size dimorphism, activity level dimorphism and in female fertility and mating behaviors. This is consistent with direct sex specific regulation of the pathway, but the targets of this regulation and the downstream effects on expression in each sex are unknown. To understand how sex differences shape the regulatory effects of the insulin signaling pathway, we examined similarities and differences in the effect of perturbation of the pathway on gene expression in adult males and females. Expression of a dominant-negative InR transgene (InRDN) was driven by actin-5C-GeneSwitch controlled GAL4 in males and females. Expression was assessed by RNA-seq in replicate for each sex expressing InRDN and for genetically matched controls. By integrating perturbation data with genomic signatures of sex specific regulation, we are able to shed light on how the InR pathway is sex specifically regulated and on the indirect effects of that regulation.