Polyamines are small cationic molecules found in all organisms and are essential in growth and proliferation. Intracellular polyamine concentrations are maintained by a combination of biosynthesis, transport, and degradation. Biosynthesis and degradation have been well studied, but even a basic understanding of the transport system remains elusive. Previously our lab identified a membrane-associated P-type ATPase with six protein isoforms necessary for polyamine transport using an in vivo RNAi approach. Current work looks to build on prior work by identifying proteins in complex with the P-type ATPase through Tandem Affinity Purification (TAP) and Tandem Mass Spectroscopy (MS/MS). The description of components involved in transport will allow us to characterize a poorly understood biological system relevant to all organisms.