The mechanisms that regulate the transduction of noxious stimuli and generation of appropriate behavioral responses in Drosophila melanogaster are not fully understood. In larvae, Class IV multidendritic neurons are highly branched sensory neurons that are responsible for detecting noxious chemical, thermal, or mechanical stimuli and generating appropriate behavioral responses. Recent studies have demonstrated involvement of Wnt signaling in regulating nociception and the development of chronic pain in vertebrate models, but the underlying cellular and molecular mechanisms are still not understood. In order to better understand the roles of Wnt signaling in Drosophila nociception, I have selected 77 Wnt signaling-related genes and obtained UAS-RNAi lines for each from the Drosophila TRiP collection for an RNAi screen for nociception defects. I have crossed these RNAi lines with flies carrying the Class IV-specific ppk-GAL4 driver and tested the larvae progeny with a well-established thermal nociception assay. We found the Wnt RNAi behavior phenotypes can be divided into three classes: 1) hypersensitivity to noxious stimuli; 2) insensitivity to noxious stimuli; 3) no change in response to noxious stimuli. These phenotypic categories are established by comparing the response latencies of RNAi animals to the latency of a transgenic control strain. Once candidate genes that produce significant hypersensitivity or insensitivity to thermal stimuli have been identified based on their behavioral phenotypes, I can then further analyze these genes to identify the cellular and molecular mechanisms of how they control nociceptor development and function maintain appropriate sensitivity to noxious stimuli. I have identified the protein tyrosine kinase 7 homolog, off track2, as a promising candidate for further analysis based on its reduced sensitivity to noxious thermal stimuli and previously established role in Wnt2 signaling. This further analysis consists of 1) genetic conformation with additional RNAi lines and genetic mutants; 2) gain and loss of function studies and epistasis analysis; 3) functional studies to determine the role of off track2 in Class IV neuron morphological development and electrical activity.