Precise spatial and temporal activation of target genes at specific tissues or cells are critical in the study of developmental processes in vivo. Currently, various chemical and genetic methods of gene activation used in nematodes lack precise spatiotemporal specificity. We adopted a light dependent gene activation system for a more precise and controlled gene activation. Using light-sensitive protein dimerization of Arabidopsis cryptochrome 2 and the bHLH protein CIB1, the CRY2-CIB system activates gene expression through the interaction of two fusion proteins, CRY2-LexA DNA binding domain and CIB-VP16. In the presence of blue light, the two fusion proteins bind and activates the transcription of target genes under the LexA promoter. To track activation of target genes, we created a LexA activated target gene followed by SL2 splice site linked to mCherry florescent reporter. Preliminary results showed positive target gene activation in transgenic following a global exposure of blue light. Further studies will be done to observe target gene activation in a tissue specific manner in various nematode developmental stages.