Drosophila is a key model system in the genetics of ethanol sensitivity and tolerance. Ethanol sensitivity is defined as the overall sensitivity to the effects of ethanol exposure, ultimately resulting in sedation. Tolerance occurs when sensitivity is reduced after one or more initial ethanol exposure/s. Molecular studies have identified genes involved in sensitivity and tolerance in Drosophila melanogaster, with the aim of understanding their role in alcoholism and alcohol related diseases. However, ethanol is an important abiotic factor for many species of Drosophila and ethanol sensitivity and tolerance vary across the genus. In some species, including in D. melanogaster, the response to ethanol is also sexually dimorphic. Ethanol sensitivity and tolerance have not been assayed for all the currently sequenced Drosophila species in both males and females, and little is known about how sexual dimorphism in sensitivity or tolerance differs across species. Here we report the results of systematic sedation assays for sensitivity and tolerance in males and females across a diverse group of sequenced species of Drosophila. These experiments place diversity of ethanol sensitivity and tolerance, including sexual dimorphism, in a phylogenetic context. This will allow for a more complete understanding of how phenotypic diversity in the ethanol response is related to differences in the regulatory response to ethanol, in each sex.