Epithelial cell cytokinesis is coupled to the formation of a new septate junction between the daughter cells, thereby preserving the integrity of the permeability barrier function during proliferation. Here, using live confocal imaging, we studied the formation of bicellular and tricelluar septate junctions during cytokinesis in the notum of Drosophila pupa. We found that the septate junctions are maintained during cytokinesis, and the assembly of the new septate junctions relies on neighboring epithelial cells ingressing in between the two daughter cells, binding and following the movement of the midbody. The detachment of the neighboring cells from the midbody coincides with the maturation of the bicellular and tricellular septate junctions. Our studies reveal how epithelial cells establish new septate junctions during cytokinesis while maintaining septate junctional seals.