The elimination of paternal organelles following fertilization is well conserved amongst metazoans, but the mechanism of organelle selection and process execution is not well understood. Previous studies conducted on Caenorhabditis elegans reported that the ubiquitin and autophagy pathways are involved in the elimination of paternal organelles. The current working model for the removal of paternal organelles in C. elegans is that membranous organelles (MOs) are ubiquitinated and cluster together with paternal mitochondria and together they are discarded through autophagy. Two different types of ubiquitin chains are found on the MOs in newly fertilized embryos. Lysine 48 chains are found only during meiosis I and lysine 63 chains persist through all stages of meiosis and mitosis. These findings indicate that two different ubiquitination events are taking place during the tagging of paternal organelles. We have used transgenic nematodes with a GFP::Ub tag in the germline and RNAi to individually knockdown ubiquitin conjugating enzymes (UBC) to establish which UBC(s) is responsible for ubiquitinating the paternal organelles. The two different types of ubiquitin chains present on MOs suggest multiple ubiquitin events. Therefore, UBC combination knockdowns were also screened. The screen revealed ubc-18/ubc-16 and ubc-18/ubc-13/ubc-2 as the combinations that reduced the presence of GFP:Ub on MOs. Interestingly, ubc-18 appears in both of our hits. Therefore, we took a closer look at WY34 (ku354) ubc-18 mutants and stained for K48 and K63 ubiquitin chains. K63 chains were not affected by the absence of ubc-18. However, K48 chains were absent in the WY34 embryos. These results indicate that ubc-18 is a key player in initial ubiquitination of paternal organelles. .