Meiosis is a specialized cell division in sexual reproduction which produces the haploid gametes from diploid precursor cells. Two separated stages are involved in this process – meiosis I and meiosis II. Meiosis I is considered as a reductional division since the homologous chromosomes segregate to opposite spindle poles while equational sister chromatids separation takes place in meiosis II. Accurate chromosome segregation enable the genetic fidelity and prevent the generation of aneuploidies. Spindle assembly checkpoint (SAC) is a surveillance mechanism to ensure completion of bipolar alignment before chromosome segregation and the role of SAC in meiosis has not been fully characterized. Rough Deal is a protein involved in SAC and responsible for the recruitment of other SAC-related proteins onto kinetochores during cell division. In mitosis, mutations in rod lead to stretched chromatid arms, anaphase lagging chromatids and chromosome mis-segregation while null mutations are lethal. In present study, we took advantage of a fully viable meiosis-specific rod allele to assess the role of rod in male meiosis. Genetic cross experiments exhibited high rates of sex chromosome nondisjunction in both stages of meiosis. Although chromosome morphology, conjunction and cohesion were normal throughout prophase I, the loss of centromere cohesion on autosomes appeared at metaphase I and later stages based on anti-CID immunostaining and FISH analysis. Cytological data in anaphase I revealed additional abnormalities, such as uneven chromosome segregation and lagging chromosome, and equational sex chromosome segregation was also observed at this stage. Future studies will concentrate on how rod mutation affect centromere cohesion and chromosome segregation in Drosophila meiosis. .