High resolution melting analysis together with Real time PCR was tested for quick identifications of cellobiohydrolase I (cbhI), Ketosynthase, beta tubulin and two ribosomal proteins (RpS29 and RpS26) in eight strains of Sordaria fimicola (S1, S2, S3, N5, N6, N7, SW17.2 and SW92.1). HRM analysis successfully reported these (RpS29 and RpS26) genes in Sordaria fimicola first time. All strains of Sordaria fimicola were authenticated by targeting ITS2 region. Above mentioned proteins were targeted to predict three dimensional structures based on various post translational modifications (PTMs). Post translational modifications are very important to understand the regulatory mechanisms of cytoplasmic and nuclear proteins. Different post translational modifications (PTMs) such as phosphorylation, glycosylation, acetylation, and methylation control the structure, function regulation of proteins. In this work the role of different modifications and their interplay, is investigated by using various bio-informatics tools.