PgmNr D1508: The activation of new lawc transcripts after the homologous long double-stranded RNA treatment in Drosophila.

Authors:
O. Simonova; R. Cherezov; J. Vorontsova; I. Mertsalov; D. Kulikova


Institutes
Koltzov Institute of Developmental Biology, Moscow, RU.


Keyword: lncRNAs

Abstract:

The increasing of gene expression by small RNAs and long non-coding RNAs homologous to promoter or 3'-UTR gene region (RNA activation, RNAa) is opposite to phenomenon of RNA-interference (RNAi). Being opened in the human cell culture (Li et al., 2006) RNAa is considered to be an evolutionary conserved, but in Drosophila it has not been yet described although it is one of the most convenient model organism to study. Early we have discovered that long double stranded RNAs homologous to leg-arista-wing complex (lawc) gene Open Reading Frame caused activation of lawc gene expression in Drosophila melanogaster (Cherezov et al., 2013). Now we confirmed our finding in Drosophila  Schneider 2 (S2) cell culture. We have cloned activated lawc RNAs and its subsequent analysis revealed that the activated RNAs were new long non-coding lawc transcripts. The mechanism of new lawc transcripts activation remained unclear but we proposed that in normal conditions its expression could be suppressed by a new set of microRNAs previously unstudied (Schertel et al., 2012). .