The giant unicellular ciliate Stentor coeruleus has the ability to fully regenerate after being cut in half, in a way that perfectly preserves cell polarity and structure. This regenerative ability has made it a classical model system for studying regeneration at the cellular level. So far, however, the molecular details behind this incredible phenomenon have remained largely unstudied. Recently, our laboratory has developed a system for RNAi knockdown of Stentor genes, and additionally sequenced the Stentor coeruleus genome. Interestingly, not only do Stentor’s introns appear to possess the smallest average intron length of any organism described to date at 15 bp, but Stentor also seems to use the standard genetic code, unlike other ciliates. We wish to understand how the regeneration process is coordinated at the molecular level. Some of the details of the regeneration process parallel the events of cell division, and thus we additionally wish to understand whether the cell co-opts certain cell division signaling pathways for regeneration.To identify candidates for RNAi knockdown we analyzed the kinome of Stentor by looking for protein kinase domains among the predicted protein coding genes. Stentor was found to encode more than 2000 kinases, making up 6% of the total protein coding genes. Many of these consist of expansions in mitotic kinase families such as PLKs, NDRs, and NEKs. There are also expansions of families absent in animals and yeast; over 12% of the kinome consists of the calcium-dependent CDPK family, originally identified in plants. We also analyzed additional protein domains found on kinase genes in Stentor, revealing a few novel domain architectures. The most notable example is an adenylate kinase fused to a calcium-dependent protein kinase, with a large region in between containing a AAA+ ATPase and other protein domains. RNAi screening of kinase genes is ongoing, and will ultimately reveal which of these kinases help to coordinate the many different precisely timed cellular events required for successful regeneration. In the future, a better understanding of the mechanisms behind single cell regeneration will have important implications for basic biology as a whole, and will reveal how these single cells can establish and maintain their polarity and cortical organization with such a high degree of precision.