Domesticated soybeans (Glycine max) are an important source of protein for livestock in the Americas and an increasingly popular food source for humans around the world. Soybeans were originally domesticated 6,000-9,000 years ago in China and share a common ancestor with wild soybeans (G. soja). Both species share the same number of chromosomes (2n = 40) and crosses between the two species generate fertile hybrids. The two species exhibit distinct phenotypes with most modern domesticated G. max having large yellow seeds with upright plants and most G. soja having small black seeds with weedy vines. The past eighty years of soybean breeding in the United States has led to significant increases in yield but at the cost of producing less protein per seed. Furthermore, there is a growing demand to produce soybean seeds with increased cysteine, methionine, and lysine content in order to optimize monogastric animal growth. Eighty G. soja soybean lines, representing the broadest possible diversity of wild soybean germplasm, were screened for seed protein and amino acid composition. We identified a handful of elite G. soja lines with high seed protein and favorable amino acid profiles. The sixth generation of two populations derived from elite G. soja crosses with G. max were subsequently evaluated for domestication traits and seed protein. Preliminary results suggest protein content is linked or overlapping with loci controlling soybean domestication. These hybrid populations, therefore, provide clues to locate genetic determinants of protein and amino acid composition in domesticated soybeans.